Ahmed,, A., El-Deeb, F., Hammad, A., Nematalla, K. (2006). RP-HPLC ‘• EVALUATION FOR SOME INSULIN INTERACTIONS. Journal of Food and Dairy Sciences, 31(12), 7787-7795. doi: 10.21608/jfds.2006.236903
A. I. S. Ahmed,; F. El-Deeb; A. A. Hammad; Kh. M. Nematalla. "RP-HPLC ‘• EVALUATION FOR SOME INSULIN INTERACTIONS". Journal of Food and Dairy Sciences, 31, 12, 2006, 7787-7795. doi: 10.21608/jfds.2006.236903
Ahmed,, A., El-Deeb, F., Hammad, A., Nematalla, K. (2006). 'RP-HPLC ‘• EVALUATION FOR SOME INSULIN INTERACTIONS', Journal of Food and Dairy Sciences, 31(12), pp. 7787-7795. doi: 10.21608/jfds.2006.236903
Ahmed,, A., El-Deeb, F., Hammad, A., Nematalla, K. RP-HPLC ‘• EVALUATION FOR SOME INSULIN INTERACTIONS. Journal of Food and Dairy Sciences, 2006; 31(12): 7787-7795. doi: 10.21608/jfds.2006.236903
RP-HPLC ‘• EVALUATION FOR SOME INSULIN INTERACTIONS
1Dept. of Special Food and Nutrition ETRI, ARC, Giza
2Fac Spe Edu. Mansoura Univ.
Abstract
In present study the RP-HPLC characteristics were used for the evaluation of
insulin interactions. These characters such as retention time (RT), area under the
peak (AP), peak height (PH), and peak width (PW) were all examined. Free or
uncontrollable cholesterol (UC) and fenugreek extract (FG) have been chosen to be
the fat and food tested metabolites. It seems, as a general conclusion, that food
fragment is considered to be preventive agent more than to be a treatment,
meanwhile, it is a pH value dependent interaction, i.e. more acid or base in blood is
less responded by food metabolites.
The most important achievement in present study was the capability ol the
technique to specifically determine the insulin interaction with UC. The new sharp < /p>
analytical measurement is centered in a new peak of RT of more than 4 min for the
protein only connected with the presence of UC. The new peak for the hormone is
another one than its preceding original peak of about 2 min. The other mentioned
HPLC characters sensitivity to these particular interactions was determined and
separately discussed.
A modified technique based on this fast and simple assay• may add a
nutritional and medical significance to the field of cholesterolemia, hence insulin
interaction becomes one of the most important molecular biology diagnostic test that
constitute cholesterolernia cluster factors. These reactions were strongly recorded in
both chromatographic and chromophorici elements, especially AP, PW and PH.
Moreover, the HPLC data has been supported by some spectrophotometric
techniques.
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