Nawar, M. (2006). SDS-PAGE POLYACRY_LAMIDE GEL ELECTROPHORESIS IS A RELIABLE METHOD FOR DETECTION OF MASTITIC MILK. Journal of Food and Dairy Sciences, 31(11), 7257-7266. doi: 10.21608/jfds.2006.236892
M. A. Nawar. "SDS-PAGE POLYACRY_LAMIDE GEL ELECTROPHORESIS IS A RELIABLE METHOD FOR DETECTION OF MASTITIC MILK". Journal of Food and Dairy Sciences, 31, 11, 2006, 7257-7266. doi: 10.21608/jfds.2006.236892
Nawar, M. (2006). 'SDS-PAGE POLYACRY_LAMIDE GEL ELECTROPHORESIS IS A RELIABLE METHOD FOR DETECTION OF MASTITIC MILK', Journal of Food and Dairy Sciences, 31(11), pp. 7257-7266. doi: 10.21608/jfds.2006.236892
Nawar, M. SDS-PAGE POLYACRY_LAMIDE GEL ELECTROPHORESIS IS A RELIABLE METHOD FOR DETECTION OF MASTITIC MILK. Journal of Food and Dairy Sciences, 2006; 31(11): 7257-7266. doi: 10.21608/jfds.2006.236892
SDS-PAGE POLYACRY_LAMIDE GEL ELECTROPHORESIS IS A RELIABLE METHOD FOR DETECTION OF MASTITIC MILK
Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Egypt.
Abstract
lndivrdual normal and mastitic milk samples were collected from healthy and
udder infected cows Milk samples were subjected to polyacrylamide gel
electrophoretic analysis in order to monitor any conSiderable changes in milk proteins
At the same time, samples were analysed for the concentration of immune system
factors which increase in milk after infection of the udder These factors include
immunoglobulinG (lgG). lysozyme (L2) and iactoferrin (LF)
The obtained results revealed that ccncentrations of IgG. L2 and LF were
markedly increased with mastitis incidence and increase in concentrations was
mastitis-degree dependent Since in first degree mastitic milk the concentrations of
L2. LF and IgG were increased by 1 2. 1 5 and 1 3 times, respectively. than in normal
milk. Eut in second and third degrees their concentrations increased by i 3 7 6 71
t4], 13 3) and ( 1 5. 1 8) times for L2. LF. and lgG respectively This result revealed
that among the immune proteins, LF and L2 not lgG were highly affected by
inflammation of the udder The electrophoretic patterns of normal and mastitic milk
were completely distrngurshable, Since the intensities of B- and ds—casein bands were
markedly decreased in mastitic milk and the decrease was mastitis degree
dependent. On the contrary the intensities of Bovrne Serum Albumin (BSA), IgG ard
LF band were obviously increased in mastitic milk and were positive correlated to
degree of mastitis. Meanwhile, some unique peptides were revealed in patterns of
second and third degrees of mastitic milk. not present in both
normal and first degree mastitic milk They have molecular weights of 21 2
49.8. 54 2 7'8 4 and 82 2 kDa This means that they are related to the intenSity of
udder inflammation, therefore these peptides can be used as biomarkers for detection
of mastitic milk. From these findings it can be concluded that the differentiation not
only between normal and mastitic milk but also for detecting the degree of mastitis.
can be ea5ily achieved,
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