Abd EI Aal,, F., Rofael, S. (2002). NON CONVENTIONAL METHODS FOR EXTRACTING PROTEIN FROM COWPEA LEAVES: 1- OPTIMUM CONDITIONS FOR PROTEIN ISOLATION. Journal of Food and Dairy Sciences, 27(7), 4763-4769. doi: 10.21608/jfds.2002.255916
Fatma EI-Zahraa A. Abd EI Aal,; Salwa O. Rofael. "NON CONVENTIONAL METHODS FOR EXTRACTING PROTEIN FROM COWPEA LEAVES: 1- OPTIMUM CONDITIONS FOR PROTEIN ISOLATION". Journal of Food and Dairy Sciences, 27, 7, 2002, 4763-4769. doi: 10.21608/jfds.2002.255916
Abd EI Aal,, F., Rofael, S. (2002). 'NON CONVENTIONAL METHODS FOR EXTRACTING PROTEIN FROM COWPEA LEAVES: 1- OPTIMUM CONDITIONS FOR PROTEIN ISOLATION', Journal of Food and Dairy Sciences, 27(7), pp. 4763-4769. doi: 10.21608/jfds.2002.255916
Abd EI Aal,, F., Rofael, S. NON CONVENTIONAL METHODS FOR EXTRACTING PROTEIN FROM COWPEA LEAVES: 1- OPTIMUM CONDITIONS FOR PROTEIN ISOLATION. Journal of Food and Dairy Sciences, 2002; 27(7): 4763-4769. doi: 10.21608/jfds.2002.255916
NON CONVENTIONAL METHODS FOR EXTRACTING PROTEIN FROM COWPEA LEAVES: 1- OPTIMUM CONDITIONS FOR PROTEIN ISOLATION
Department of Hort. Crops Processing, Food Tec. Research Inst., ARC, Egypt.
Abstract
Chemical composition and some antinutrational factors of cowpea leaves (Vigna sinensis, Savi) , as well as setting up the optimum conditions for maximum protein extraction were studied. The electrophoretic properties of protein extraction were also investigated . Our results led to the following conclusions: - Cowpea leaves had a good source of leaf protein because it's content was 30.40% as crude protein. The highest extractable protein yeild at SO' C, after 90 minuets and pH 9 was found to be the optimum value for maximum recovery using distilled water in a ratio 1 :25 (w/w) ( cowpea leaves pwoder : solvent) , Most of protein fractions of cowpea leaves were focused in about 6 bands in the pH range of 3.5 - 4.7. Protein extract was preclpated according to it's iso electric point which found at pH 4.
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