NON CONVENTIONAL METHODS FOR EXTRACTING PROTEIN FROM COWPEA LEAVES: 1- OPTIMUM CONDITIONS FOR PROTEIN ISOLATION

Document Type : Original Article

Authors

Department of Hort. Crops Processing, Food Tec. Research Inst., ARC, Egypt.

Abstract

Chemical composition and some antinutrational factors of cowpea leaves (Vigna
sinensis
, Savi) , as well as setting up the optimum conditions for maximum protein
extraction were studied. The electrophoretic properties of protein extraction were also
investigated . Our results led to the following conclusions: - Cowpea leaves had a
good source of leaf protein because it's content was 30.40% as crude protein. The
highest extractable protein yeild at SO' C, after 90 minuets and pH 9 was found to be
the optimum value for maximum recovery using distilled water in a ratio 1 :25 (w/w)
( cowpea leaves pwoder : solvent) , Most of protein fractions of cowpea leaves were focused in about 6 bands in the pH range of 3.5 - 4.7. Protein extract was preclpated according to it's iso electric point which found at pH 4.

Keywords