BATCH AND REPEATED FED BATCH CULTIVATION OF Candida utilis ON CORN MEAL HYDROLYSATE FOR CELLULAR BIOMASS PRODUCTION.

Document Type : Original Article

Authors

Agric. Microbiol. Res. Dept., Soils, Water and Environ. Res. Inst., Agric. Res. Center, Giza- Egypt.

Abstract

A Fermentation medium based on corn meal hydrolysate was developed for the production of single cell protein (SCP)using the yeast strain Candida  utilis  . The corn meal was gelatinized for 1h at 80oC then liquefied with a thermostable  α- amylase at 70oC. The liquefied starch was saccharified with glucoamylase at 55 oC for two hours . Over 98% of the starch was hydrolyzed, about 80% of the hydrolysate was glucose . The hydrolysate was formulated in a suitable medium for supporting the requiste growth rate and yield of Candida utilis . The growth parameters of Candida utilis grown on corn meal hydrolysate in a simple batch fermentation mode are presented .A strategy for utilization of repeated fed batch fermentation was demonstrated. The data showed that the repeated fed batch mode was a suitable fermentation technique for biomass production.

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