Document Type : Original Article
                            
                        
                                                    Authors
                            
                                                            
                                                                            1
                                                                        Food Science and Techno!. Dept., Fact. of Agric., Tanta, Tanta nlv.                                
                                                            
                                                                            2
                                                                        Home Economics Dept., Fact. of Specific Education, Tanta Uni ., Egypt.                                
                            
                                                                            
                        
                        
                            Abstract
                            Soybean was de hulled. soaked. autoclaved and fermented by two species of 
 Rhizopus namely. R. o/igosp < /em>orus and R. arrhlzus .to produce tempe. Chemical. 
 nutritional. microbiological and organoleptical evaluation of soybean lempe groducts 
 were investigated. The best products were obtained after 26 and 30 hrs at ~1. 32·C 
 by R. oligosporus end R. entuzus. respectively. II was noticed that. soaking and 
 autoclaving processes decreased significantly (P S 0.05) contents of ether extract, 
 ash, total carbohydrate, lotal solids, soluble solids. insoluble solids. TP, minerals. 
 number of yeasts and lactic acid bacteria. On the contrary, moisture, crude protein 
 and NPN contents were increased signifICantly (P ~ 0.05) in soaked-au oelaved 
 soybean grits, while crude fiber content and total bacterial spores count did not 
 change in the same grits. However, fermentation process either by R. o/lgosp < /em>orus or 
 R arrhizus elevated Significantly (P s 0.05) contents of moisture. crude protein. NPN. 
 crude fiber, soluble solids, counts of lactic acid bacteria and total bacteria spores. 
 Conversely, ether extract, total carbohydrate, total solids, insoluble solids and TP 
 contents were diminished significanlly (P !> 0.05) in tempe products. Fe and K 
 contents increased slightly after fermentation process, but Ca, Cu and Na contents 
 decreased slightly after this process. Conlents of Mg, Mn, Zn and yeasts cou~t did not 
 alter appreciably after fermentation. Slight or no changes in the essential amino acids 
 content were occurred as a result of tempe production. The most of essential amino 
 acids were close to the amounts needed for protein balance according to t e FAO I 
 WHO (1973)' reference protein. Sulfur-conlanirig amino acids gave the lowest score, 
 although they were increased by fermentation. Chemical scores of essent (amino 
 acids for lempe produced by R. arrhiws were higher than those for tempe fermented 
 by R.o/igosporus with the exception of leucine. Data also indicated th t tempe 
 produclion process improved significantly (P 50.05) the protein digestibility, C-PER 
 and biological value. Moreover. trypsin inhibitor and phytic acid conte ts were 
 diminished significantly (P s 0.05) as a result of tempe production. Finally sensory 
 evaluation established that all fried tempe products were accepted.
                        
                        
                        
                                                    Keywords